p300/Sp1-Mediated High Expression of p16 Promotes Endothelial Progenitor Cell Senescence Leading to the Occurrence of Chronic Obstructive Pulmonary Disease

Figure 2

High expression of p16 in EPCs promoted cell cycle arrest and apoptosis. (a) Peripheral blood EPCs were isolated from nonsmoking non-COPD, smoking non-COPD, and smoking COPD patients, and cell proliferation was detected by CCK8. (b) Peripheral blood EPCs of smoking COPD patients were isolated, p16 was knocked down by siRNA, and cell proliferation was detected by CCK8. (c) Peripheral blood EPCs from nonsmoking non-COPD were treated with 5% CSE, p16 was knocked down by siRNA, and cell proliferation was detected by CCK8. (d) Peripheral blood EPCs were isolated from nonsmoking non-COPD, smoking non-COPD, and smoking COPD patients, and cell cycle was detected by flow cytometry. (e) Peripheral blood EPCs from smoking COPD patients were isolated, siRNA knocked down p16, and cell cycle was detected by flow cytometry. (f) Peripheral blood EPCs from nonsmoking non-COPD patients were treated with 5% CSE, p16 was knocked down by siRNA, and cell cycle was detected by flow cytometry.

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