p300/Sp1-Mediated High Expression of p16 Promotes Endothelial Progenitor Cell Senescence Leading to the Occurrence of Chronic Obstructive Pulmonary Disease

Figure 3

CSE-mediated high expression of p16 promoted cell senescence. (a) Peripheral blood EPCs from nonsmoking non-COPD patients were treated with 5% CSE, p16 was knocked down by siRNA, and the proportion of senescence cells was counted by β-galactosidase staining. (b) Peripheral blood EPCs from nonsmoking non-COPD patients were treated with 5% CSE, p16 was knocked down by siRNA, and the expression of Lamp1 was detected by immunofluorescence. (c) Peripheral blood EPCs from nonsmoking non-COPD patients were treated with 5% CSE, p16 was knocked down by siRNA, and the expressions of senescence-related genes Col1a1, MMP3, MMP13, and Pal1 were detected by RT-PCR. (d) Peripheral blood EPCs from nonsmoking non-COPD patients were treated with 5% CSE, p16 was knocked down by siRNA, and the expressions of senescence-related genes Col1a1, MMP3, MMP13, and Pal1 were tested by Western Blot. , , and .

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