p300/Sp1-Mediated High Expression of p16 Promotes Endothelial Progenitor Cell Senescence Leading to the Occurrence of Chronic Obstructive Pulmonary Disease

Figure 5

p300/Sp1 regulated p16 transcriptional activity. (a) Different amounts of p300 were overexpressed in EPCs, and the activity of the p16 promoter was detected by luciferase. (b) Different amounts of Sp1 were overexpressed in EPCs, and the activity of the p16 promoter was detected by luciferase. (c) p300/Sp1 were knocked down in EPCs, and the activity of the p16 promoter was detected by luciferase. (d) p300/Sp1 were overexpressed or knocked down in EPCs, and the expression of p16 was detected by RT-PCR. (e) p300/Sp1 were overexpressed or/and knocked down in EPCs, and the expression of p16 was tested by Western Blot. (f) p300 was overexpressed or/and knocked down in EPCs, and the level of histone H4 acetylation in the p16 promoter region was detected by ChIP assay. (g) Sp1 was overexpressed or/and knocked down in EPCs, and the level of histone H4 acetylation in the p16 promoter region was detected by the ChIP assay. All the EPCs used in this figure were from nonsmoking non-COPD patients. , , and .

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