p300/Sp1-Mediated High Expression of p16 Promotes Endothelial Progenitor Cell Senescence Leading to the Occurrence of Chronic Obstructive Pulmonary Disease

Figure 6

Low expression of p300/Sp1 inhibited CSE-mediated cell senescence. (a) Peripheral blood EPCs were treated with 5% CSE, p300/Sp1 were knocked down by siRNA, and the proportion of senescence cells was counted by β-galactosidase staining. (b) Peripheral blood EPCs were treated with 5% CSE, p300/Sp1 were knocked down by siRNA, and the expression of Lamp1 expression was detected by immunofluorescence. (c) Peripheral blood EPCs were treated with 5% CSE, p300/Sp1 were knocked down by siRNA, and cell cycle distribution was detected by flow cytometry. (d) Peripheral blood EPCs were treated with 5% CSE, p300/Sp1 were knocked down by siRNA, and the expressions of senescence-related genes Col1a1, MMP3, MMP13, and Pal1 were detected by RT-PCR. (e) Peripheral blood EPCs were treated with 5% CSE, p300/Sp1 were knocked down by siRNA, and the expressions of senescence-related genes Col1a1, MMP3, MMP13, and Pal1 were tested by Western Blot. (f) Peripheral blood EPCs were treated with 5% CSE, p300/Sp1 were knocked down by siRNA, p16 was overexpressed, and the expressions of senescence-related genes Col1a1, MMP3, MMP13, and Pal1 were detected by RT-PCR. (g) Peripheral blood EPCs were treated with 5% CSE, p300/Sp1 were knocked down by siRNA, p16 was overexpressed, and the expressions of senescence-related genes Col1a1, MMP3, MMP13, and Pal1 were tested by Western Blot. All the EPCs used in this figure were from nonsmoking non-COPD patients. , , and .

(a)

(b)

(c)

(d)

(e)

(f)

(g)