Effect and Mechanism of TL1A Expression on Epithelial-Mesenchymal Transition during Chronic Colitis-Related Intestinal Fibrosis

Figure 4

TL1A promotes EMT in the DSS-induced intestinal fibrosis model. (a) Immunofluorescence staining of colon tissue sections from wild type (WT) and transgenic (Tg) mice probed with antibodies against E-cadherin (green) and FSP1 (red) (400x). E-cadherin⁺FSP1⁺ cells (yellow; reflects EMT). Nuclei are stained with DAPI (blue). (b) Immunohistochemical staining of β-catenin in colon tissues of mice groups (400x). (c–g) Western blot analysis of total protein of β-catenin, E-cadherin, α-SMA, and FSP1 in intestinal tissue, normalized with GAPDH. (h–j) RT-PCR analysis of E-cadherin, α-SMA, and FSP1 mRNA levels in the indicated groups. Data were given as deviation (SD). As compared to the control group: , , and .

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