Ellagic Acid Alleviates Rheumatoid Arthritis in Rats through Inhibiting MTA1/HDAC1-Mediated Nur77 Deacetylation

Figure 4

MTA1 knockdown inhibited proliferation, inflammation, and oxidative stress and promoted apoptosis by downregulating HDAC1 in MH7A cells. TNF-α-treated MH7A cells were transfected with si-MTA1 alone or together with pcDNA-HDAC1. (a–c) The protein expression of MTA1 and HDAC1 was detected by Western blot analysis. (d) MH7A cell proliferation was measured by CCK-8 assay. (e, f) Flow cytometry was performed to measure MH7A cell apoptosis. (g, h) The levels of IL-6 and IL-1β in cell culture supernatant were detected by ELISA. (i, j) The levels of SOD and MDA in MH7A cells were examined by commercial kits. Data from at least three replicate experiments were presented as . . .

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