Ellagic Acid Alleviates Rheumatoid Arthritis in Rats through Inhibiting MTA1/HDAC1-Mediated Nur77 Deacetylation

Figure 5

EA inhibited HDAC1-mediated Nur77 deacetylation in MH7A cells. (a) ChIP was employed to detect the enrichment of HDAC1 on the Nur77 promoter region in MH7A cells after HDAC1 overexpression. (b–d) MH7A cells we transfected with pcDNA-HDAC1 alone or together with treatment with 5 μM HDAC1 inhibitor SAHA, the mRNA and protein expression of Nur77 was detected by RT-qPCR and Western blot analysis, respectively. (e) TNF-α-treated MH7A cells were treated with 50 μM EA; ChIP was employed to detect the enrichment of HDAC1 in the Nur77 promoter region in MH7A cells. (f–h) The mRNA and protein expression of Nur77 was detected by RT-qPCR and Western blot analysis, respectively. Data from at least three replicate experiments were presented as . . .

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