Effect of Short-Term Tacrolimus Exposure on Rat Liver: An Insight into Serum Antioxidant Status, Liver Lipid Peroxidation, and Inflammation

Figure 1

(a) Effect of short-term exposure of Tacrolimus on histopathological alterations in rat liver sections of control, 6, 12, 24, and 48 h treatment groups. PAS-D staining of liver control group showing intact liver architecture with almost no globules, while treatment groups indicate derangement of hepatocytes and presence of globules (arrows) due to collagen digestion (400x). Scale bar used is 50 μm. (b) Effect of short-term exposure of Tacrolimus on histopathological alterations in rat liver sections of control, 6, 12, 24, and 48 h treatment groups. Reticulin staining of control group showing intact liver architecture with normal reticulin fibers, while treatment groups indicate derangement of hepatocytes and collapsed reticulin network (black gray color) (400x). Scale bar used is 50 μm. (c) Effect of short-term exposure of Tacrolimus on PCNA immunostaining in rat liver tissue sections of control, 6, 12, 24, and 48 h treatment groups (400x). Scale bar used is 50 μm. Degree of brown staining shows intensity of immune reaction. (d) IRS of PCNA in all experimental groups was scored and data was analysed using one-way ANOVA. Data is represented as (). , , and . (e) Effect of short-term exposure of Tacrolimus on CK-7 immunostaining in rat liver tissue sections of control, 6, 12, 24, and 48 h treatment groups (400x). Scale bar used is 50 μm. Degree of brown staining shows intensity of immune reaction. (f) IRS of CK-7 in all experimental groups was scored and data was analysed using one-way ANOVA. Data is represented as (). , , . (g) Effect of short-term exposure of Tacrolimus on DNA damage in rat liver sections of control, 6, 12, 24, and 48 h treatment groups depicted by TUNEL assay staining (400x). Scale bar used is 50 μm. (h) TUNEL-positive cells in all experimental groups were counted using ImageJ, and data was analysed using one-way ANOVA. Data is represented as (). , , and .

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