Metformin and Probiotics Interplay in Amelioration of Ethanol-Induced Oxidative Stress and Inflammatory Response in an In Vitro and In Vivo Model of Hepatic Injury

Figure 6

(a) Microscopic images of Oil red O staining showing the results of (A) control, (B) 100 mM ethanol control, (C) 100 mM ethanol+10 μl/ml probiotic V, (D) 100 mM ethanol+1 mM Met, and (e) 100 mM ethanol+10 μl/ml probiotic V and 1 mM Met combination exposed HepG2 cells showed increased lipid accumulation. The above images are representative of three different experiments at the 10x objective. (b) Spectrophotometric analysis results of lipid accumulation in HepG2 cells by Oil red O extraction method. Ethanol-exposed HepG2 cells cotreated with probiotic V and Met showed decrease lipid accumulation when compared to ethanol-exposed cells. Results are ( individual experiments). Statistical significance was assessed by one-way ANOVA followed by Tukey post hoc test. Statistical analysis: ^#### compared with control group; and compared with ethanol group; ^AA,BB compared with probiotic V and Met combinatorial group.

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