Metformin and Probiotics Interplay in Amelioration of Ethanol-Induced Oxidative Stress and Inflammatory Response in an In Vitro and In Vivo Model of Hepatic Injury

Table 1

The list of Homo sapiens primers used for quantification of mRNA expression levels in qRT-PCR.

Sr. No.	Name of the gene	Forward primer sequence	Reverse primer sequence
1.	18S	ACGGAAGGGCACCACCAGGA	CACCACCACCCACGGAATCG
2.	iNOS	CCCTTCCGAAGTTTCTGGCAGCAGC	GGCTGTCAGAGAGCCTCGTGGCTTTGG
3.	CYP2E1	AGGGTACCATGTCTGCCCTCGGAGTGA	ACAATTTGAAAGCTTGTTTGAAAGCGG
4.	TNF-α	CCCTCACACTCAGATCATCTTCT	GCTACGACGTGGGCTACAG
5.	IL-10	ACTGCTAACCGACTCCTTA	TAAGGAGTCGGTTAGCAGT
6.	HO-1	AAGCCGAGAATGCTGAGTTCA	CGGGTGTAGATATGGTACAAGGA
7.	IL-6	GACAACTTTGGCATTGTGG	ATGCAGGGATGATGTTCTG
8.	CHOP	GAAAGCAGAAACCGGTCCAAT	GGATGAGATATAGGTGCCCCC
9.	Grp78	GAAACTGCCGAGGCGTAT	ATGTTCTTCTCTCCCTCTCTCTTA
10.	TLR-4	GATTGCTCAGACATGGCAGTTTC	CACTCGAGGTAGGTGTTTCTGCT AA
11.	Nrf-2	TGCCCCTGGAAGTGTCAAACA	CAACAGGGAGGTTAATGATTT
12.	PPAR-γ	GGCTTCATGACAAGGGAGTTTC	AACTCAAACTTGGGCTCCATAAAG
13.	HSL	GTGCAAAGACGGAGGACCACTCCA	GACGTCTCGGAGTTTCCCCTCAG
14.	AMPK	GGGTGAAGATCGGACACTACGT	TTGATGTTCAATCTTCACTTTG
15.	SREBP-1c	CGGCGCGGAAGCTGT	TGCAATCCATGGCTCCGT
16.	ACC	CTGCTCGTGGATGAACCAGAC	GTCAGCCATCGCCCGAGC
17.	FAS	ACAGGGACAACCTGGAGTTCT	CTGTGGTCCCACTTGATGAGT