Metformin and Probiotics Interplay in Amelioration of Ethanol-Induced Oxidative Stress and Inflammatory Response in an In Vitro and In Vivo Model of Hepatic Injury

Table 3

The list of Rattus norvegicus primers used for quantification of mRNA expression levels in qRT-PCR.

Sr. No.	Name of the gene	Forward primer sequence	Reverse primer sequence
1.	18S	GTTGGTTTTCGGAACTGAGGC	GTCGGCATCGTTTATGGTCG
2.	CYP2E1	TCAATCTCT GGACCCCAACTG	GCGCTCTGCACTGTGCTTT
3.	TLR-4	TTGAAGACAAGGCATGGCATGC	TCTCCCAAGATCAACCGATG
4.	TNF-α	TCTCATTCCTGCTCGTGGCG	GGTGAGGAGCACGTAGTCGG
5.	IL-6	TTGACAGCCACTGCCTTCCC	CGGAACTCCAGAAGACCAGAGC
6.	HO-1	CAAATCCCACCTTGAACACA	CGACTGACTAATGGCAGCAG
7.	Nrf-2	CAGAGTTTCTTCGCCAGAGG	TGAGTGTGAGGACCCATCG
8.	PPAR-γ	ACGATCTGCCTGAGGTCTGT	CATCGAGGACATCCAAGACA
9.	HSL	AGTGAAAAACCCGCGGACC	TTCATCCTTCTGCCCCCTAC
10.	AMPK	GCTGTGGATCGCCAAATTAT	GCATCAGCAGAGTGGCAATA
11.	SREBP-1c	TCTGCCTTGATGAAGTGTGG	AGCAGCCCCTAGAACAAACA
12.	ACC	CCTTCTTCTACTGGCGACTGAG	TAAGCCTTCACTGTGCCTTCC
13.	FAS	CCTCAGTCCTGTTATCACCCGA	GCTGAATACGACCACGCACTA
14.	IL-10	TGCCTTCAGTCAAGTGAAGAC	AAACTCATTCATGGCCTTGTA