Metformin and Probiotics Interplay in Amelioration of Ethanol-Induced Oxidative Stress and Inflammatory Response in an In Vitro and In Vivo Model of Hepatic Injury

Table 4

Effect of probiotic V and Met on the viability of HepG2 cells. The average percentage of cell viability after respective treatment was analyzed through MTT assay. Values represent the of three individual experiments. Statistical significance was assessed by one-way ANOVA followed by Tukey post hoc test. Statistical analysis: ^#### compared with control untreated group; , , and compared with ethanol alone; ns stands for nonsignificant.

Groups	Cell viability (%)
	Control	Ethanol
Untreated	95.7	64.6^####
Probiotic V (10 μl/ml)	95.5	81.9
Probiotic V (50 μl/ml)	95.3	83.1
Probiotic V (100 μl/ml)	95.3	85.5
Met (1 mM)	94.9	82.7
Met (2 mM)	93.5	81.6
Met (3 mM)	87.5	67.9^ns
Probiotic V (10 μl/ml)+Met (1 mM)	95.5	86.8
Probiotic V (10 μl/ml)+Met (2 mM)	95.3	81.2
Probiotic V (10 μl/ml)+Met (3 mM)	88.2	69.2
Probiotic V (50 μl/ml)+Met (1 mM)	95.2	87.0
Probiotic V (50 μl/ml)+Met (2 mM)	95.2	81.8
Probiotic V (50 μl/ml)+Met (3 mM)	89.5	70.2
Probiotic V (100 μl/ml)+Met (1 mM)	95.2	87.3
Probiotic V (100 μl/ml)+Met (2 mM)	95.2	82.3
Probiotic V (100 μl/ml)+Met (3 mM)	91.3	70.5