Toll-Like Receptor 4 Mediated Oxidized Low-Density Lipoprotein-Induced Foam Cell Formation in Vascular Smooth Muscle Cells via Src and Sirt1/3 Pathway
oxLDL promoted foam cell-like phenotype in VSMCs. After oxLDL stimulation, the mRNA or protein expression of genes (Myh11 and αSma as VSMC contractile phenotype marker, Mac2, and Cd68 as foam cell marker) was detected by real-time PCR or western blot in VSMCs. The untreated group served as control (Con), and β-actin served as an internal reference gene to normalize protein expression. All of the real-time PCR results were calculated with 2-ΔΔCT method; the western blot results were calculated using grayscale value. (a) mRNA levels of Myh11, αSma, Mac2, and Cd68 were measured by real-time PCR. ( per group, results were expressed as ; (c) , as compared with the Con group). (b, c) Protein expressions of Myh11, αSma, Mac2, and Cd68 were measured by western blot ( per group, results were expressed as ; as compared with the Con group).