High-Affinity Anti-VISTA Antibody Protects against Sepsis by Inhibition of T Lymphocyte Apoptosis and Suppression of the Inflammatory Response

Figure 5

Anti-VISTA mAb reversed TNF-α-induced CD3⁺ T cell apoptosis in vitro. (a) Representative images of CD3⁺ T cell apoptosis detected by flow cytometry. Spleen T cells were enriched by using CD3-coating immunomagnetic beads and then cultured with or without the presence of MH5A antibody or isotype control. Apoptosis was induced by TNF-α (1 μg/mL) stimulation in vitro. (b) Summary data of T cell apoptosis indicated by percentage of annexin V-positive cells. MH5A reversed T cell apoptosis cultured from CLP mice with or without the stimulation. . (c) Expression of cleaved caspase-3 was determined by Western blotting assay. MH5A inhibited cleaved caspase-3 expression cultured from CLP mice with or without TNF-α stimulation.

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