Functional and Therapeutic Relevance of Rho GTPases in Innate Immune Cell Migration and Function during Inflammation: An In Silico Perspective

Figure 2

Sequence alignment and structural representation of RhoA, Cdc42, and Rac1. (a) FASTA sequence of RhoA, Cdc42, and Rac1 was retrieved from the Uniprot database, and sequence alignment was performed through the Clustal Omega tool. The length of different G-boxes (G1, G2, G3, G4, and G5) in the G domain is marked in the blue line, while the length of switch regions is highlighted in the red line. Asterisk represents the entirely conserved column; colon indicates the column where all residues have approximately the same size and hydropathy; full stop indicates the column where the size or hydropathy has been preserved in the course of evolution. (b) Structural representation of RhoA (green color, PDB: 1CXZ), Cdc42 (cyan color, PDB: 1CEE), and Rac1 (yellow color, PDB: 1E96) with their switch I and switch II region highlighted in red and insert region highlighted in magenta color. (c) Structural depiction of RhoA-PKN complex (RhoA in green color and PKN in light brown, PDB: 1CXZ), Cdc42-WASP complex (Cdc42 in cyan color and WASP in orange color, PDB: 1CEE), and Rac-p67phox complex (Rac1 in yellow color and p67phox in bright pink color, PDB: 1E96). The blue color sphere represents the Mg²⁺ ion.