Expression of the Metalloproteinase ADAM8 Is Upregulated in Liver Inflammation Models and Enhances Cytokine Release In Vitro

Figure 4

Reduction of LPS-induced cytokine expression and release in primary hepatocytes with ADAM8 knockout. (a–d) Primary murine hepatocytes were generated from wild-type (WT) mice or ADAM8 knockout (A8_KO) mice. Subsequently, cells were stimulated with or without LPS (1 μg/ml) for 24 h and analysed for mRNA expression (a) and protein expression (b) of ADAM8. The data are shown as representative blot. The band intensities were quantified by densitometry and normalised to GAPDH protein expression. The mRNA expression of TNF-α (c) and IL-6 (d) was also analysed by qPCR, and all qPCR data was normalised to the mRNA expression of Rps29. (e, f) Release of TNF-α and IL-6 in primary hepatocytes was analysed by ELISA. Quantitative data are shown as the of 3-4 independent experiments. Significant differences to the unstimulated controls are indicated by asterisks (, , and ). Differences caused by knockout of ADAM8 compared to control are indicated by hashtags (^#, ^##, and ^###).

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