MiR-6869-5p Induces M2 Polarization by Regulating PTPRO in Gestational Diabetes Mellitus

Figure 3

MiR-6869-5p regulated the proliferation of macrophage by targeting PTPRO. (a) The predicted consequential paring of miR-6869-5p and PTPRO 3’UTR. (b) The luciferase reporter assay: the activity of luciferase was significantly decreased in PTPRO wild type group compared with the PTPRO mutant type group in THP-1 macrophages treated by miR-6869-5p mimics (). (c) CCK-8: miR-6869-5p mimics inhibited the proliferation of macrophage although PTPRO was overexpressed (compared with the control group, ; compared with the PTPRO (+) group, ). (d) EdU: miR-6869-5p mimics inhibited macrophage proliferation (representative pictures of EdU assay and data of three independent experiments; compared with the control group, ; compared with the PTPRO (+) group, ).

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