Ataxin-10 Inhibits TNF-α-Induced Endothelial Inflammation via Suppressing Interferon Regulatory Factor-1

Figure 2

Effects of Ataxin-10 on the adhesion of THP-1 monocytes to TNF-α-treated HUVECs. (a, c) Photographs (×200 magnification) of PKH67 fluorescent labeled monocytes (THP-1 cells, green) adhering to HUVECs. (b, d) Quantitative data analysis revealed significant increases in adhesion of THP-1 cells to the HUVEC monolayer. The data are representative of 6 independent experiments. vs. TNF-α treated control group. (a, b) Ataxin-10 or empty vector (EV) was transfected transiently into HUVECs; 48 h later, cells were incubated with or without TNF-α (10 ng/mL) for 8 h. Then, fluorescence-labeled THP-1 cells were added to the activated HUVECs for 1 h. After being washed carefully by PBS, adherent cells were visualized, photographed, and counted. (c, d) The HUVECs transfected with si-control or si-Ataxin-10 were treated with TNF-α (10 ng/mL) for 8 h and incubated with PKH67 fluorescent labeled monocytes for 1 h. Adhesive cells were visualized, photographed, and counted as well.

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