Assessment of Allergen-Responsive Regulatory T Cells in Experimental Asthma Induced in Different Mouse Strains

Figure 6

In vitro expansion of CD4⁺IL4⁺, CD4⁺IL17⁺, CD4⁺CD25⁺Foxp3⁺, and CD4⁺IL10⁺ cell populations from lymph nodes of A/J, BALB/c, or C57BL/6 OVA-challenged mice. Cells from mediastinal lymph nodes were cultured for 72 h in the presence IL-2, IL-2 + OVA, or IL-2 + OVA + TGF-β, and subpopulations of CD4⁺IL4⁺ (a) and CD4⁺IL17⁺ (b) CD4⁺CD25⁺Foxp3⁺ (c) or CD4⁺IL10⁺ (d) were analyzed by flow cytometry. IL-10 production in the supernatant of cultured cells was detected by ELISA (e). Values represent of 4-5 animals per group. ^# and ^### as compared to the same mouse strain group that received only IL-2. ⁺⁺ and ⁺⁺⁺ as compared to the same mouse strain group that received IL-2 + OVA. ^§ and ^§§§ as compared to the same mouse strain group that received IL-2 + OVA + TGF-β. ^& as compared to the same strain between different stimulus. , , . Correlation between frequency of CD4⁺IL10⁺ cells and IL-10 production in cultures stimulated with OVA and TGF-β (f). Pseudo-, by univariate analysis.