CircN4bp1 Facilitates Sepsis-Induced Acute Respiratory Distress Syndrome through Mediating Macrophage Polarization via the miR-138-5p/EZH2 Axis

Figure 1

Expression and characterization of circN4bp1 in macrophages. (a) qRT-PCR of quantification of the circN4bp1 expression in the BALF and pulmonary macrophages isolated from lung tissue homogenates of sham and CLP mice. (b) Sanger sequencing showing the “head-to-tail” splicing of circN4bp1 in macrophages. (c) qRT-PCR of quantification of the circN4bp1 and N4bp1 mRNA expression in RAW264.7 macrophages after treatment with RNase . (d) qRT-PCR quantification of the circN4bp1 and N4bp1 mRNA expression in RAW264.7 macrophages after treatment with actinomycin D. (e) RNA FISH for circN4bp1. Nuclei were stained with DAPI. Data are presented as ; significant difference was identified with Student’s -test. ; ns: no significant.

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