CircN4bp1 Facilitates Sepsis-Induced Acute Respiratory Distress Syndrome through Mediating Macrophage Polarization via the miR-138-5p/EZH2 Axis

Figure 5

miR-138-5p mimics suppress the effect of circN4bp1 on macrophage polarization. RAW264.7 was transfected with miR-138-5p mimic with/withoutcircN4bp1 lentivirus plasmids (circN4bp1-OE) or scrambled control and then exposed to either LPS (50 ng/ml) or IL-4 (10 ng/ml) for an additional 24 h. The levels of IL-6 (a), TNF-α (b), and IL-10 (c) were measured by ELISA in the supernatants of LPS and IL-4 stimulated raw264.7 cells. (d) Representative western blot depicting raw264.7 cell lysates probed for iNOS, Arg-1, and GAPDH. (e) Expression levels of iNOS and Arg-1 were quantified by densitometry and normalized using GAPDH. All data are expressed as . ( vs. NC group, # vs. miRNC + LPS stimulated group, ^@ vs. circN4bp1-OE + LPS group, & vs. miRNC +IL-4 stimulated group, and % vs. circN4bp1-OE + IL-4 group determined by one-way ANOVA for multiple group comparisons).

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