Butyrate Inhibits Osteoclast Activity In Vitro and Regulates Systemic Inflammation and Bone Healing in a Murine Osteotomy Model Compared to Antibiotic-Treated Mice

Figure 3

Rifampicin- and levofloxacin-treated mice show reduced cecal SCFA levels and a change in gut microbiome composition compared to butyrate- and control-treated mice. The effect of butyrate on the gut microbiome, systemic immunity, and bone healing was assessed in a murine osteotomy model. (a) Experimental outline of in vivo study indicating interventions and time frame. The figure was created with http://BioRender.com/. (b) Cecal concentrations of acetate, propionate, and butyrate were measured by means of UPLC. Data shown are means () and ±SEM. , , , and . (c) DNA of murine cecal content was isolated, and 16s rRNA sequencing was performed to determine microbiome composition. Percent abundance of bacterial phyla in the murine cecum ( -treated animals, butyrate-treated animals, and control-treated animals).

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