Butyrate Inhibits Osteoclast Activity In Vitro and Regulates Systemic Inflammation and Bone Healing in a Murine Osteotomy Model Compared to Antibiotic-Treated Mice

Figure 6

Butyrate does not significantly impact bone healing outcomes while rifampicin and levofloxacin may delay bone healing in a murine osteotomy model. Bone healing outcomes of operated mice were assessed 10 days, 18 days, and 21 days (euthanasia) following osteotomy. (a) Failure load of contralateral, unoperated femora was determined by a four-point-bending test. Data shown are . (b) Procollagen type I C-terminal propeptide (PINP) in serum was determined 10 days and 21 days after osteotomy. Data shown are . (c) Bone volume was measured by μCT 21 days after osteotomy. Data shown are . (d) Quantification of calcium deposition at osteotomy site 10 days after osteotomy (calcein green injection) and 18 days after osteotomy (xylenol orange injection). Data shown are . (e) Representative fluorescent images showing calcein green and xylenol orange labelling of exposed calcium at osteotomy site. , .

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