circRNA circ_102049 Implicates in Pancreatic Ductal Adenocarcinoma Progression through Activating CD80 by Targeting miR-455-3p

Figure 10

The role of circ_102049 knockdown in cell growth, apoptosis, inflammation, invasion, and migration in PDAC cells. (a) circ_102049 expression was measured after transfection in SW1990 and Panc-1 cells by RT-PCR. (b) CCK-8 assays were used to detect cell viability of SW1990 and Panc-1 cells after transfection. (c) Numbers of clone formation were decreased when transfected with si-circ_102049_#1. (d) The apoptosis of SW1990 and Panc-1 cells examined by flow cytometry after transfection. (e) Wound healing assays were performed in SW1990 and Panc-1 cells treated with si-NC or si-circ_102049_#1. (f) Cell invasion ability of SW1990 and Panc-1 cells transfected with si-NC or si-circ_102049_#1 was evaluated by the transwell assays. (g) mRNA expression levels of TNF-α, IL-6, IL-1β, IL-8, and IL-17 were detected in SW1990 and Panc-1 cells transfected with si-NC or si-circ_102049_#1 by RT-PCR. (h) circ_102049 knockdown inhibited tumor growth in vivo. Tumor weight and volumes were significantly reduced in the si-circ_102049_#1 group compared with the si-NC group. , .

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