circRNA circ_102049 Implicates in Pancreatic Ductal Adenocarcinoma Progression through Activating CD80 by Targeting miR-455-3p

Figure 11

circ_102049 functions as a miRNA sponge for miR-455-3p. (a) The binding site for miR-455-3p and circ_102049 was analyzed with the bioinformatics tool. (b) Schematic outlining the wild-type and mutant circ_102049 luciferase plasmid. (c) Luciferase reporter assay was performed to analyze the interaction between circ_102049 and miR-455-3p in PDAC cells. (d) miR-455-3p expression was confirmed by using RT-PCR in PDAC cells. (e) Overexpression and silencing of miR-455-3p in SW1990 and Panc-1 cells via miR-455-3p mimic or miR-455-3p inhibitor, respectively. (f) circ_102049 expression was detected in transfected PDAC cells with miR-455-3p mimic or miR-455-3p inhibitor, respectively. (g) miR-455-3p expression was measured in SW1990 and Panc-1 cells that were transfected with si-NC or si-circ_102049_#1. , .

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