circRNA circ_102049 Implicates in Pancreatic Ductal Adenocarcinoma Progression through Activating CD80 by Targeting miR-455-3p

Figure 12

circ_102049 regulates CD80 expression in PDAC cells by sponging miR-455-3p. (a) A predicted binding site of miR-455-3p within the CD80 3-UTR. (b) Luciferase reporter assays were employed to assess the interaction between CD80 3-UTR and miR-455-3p. (c) circ_102049 knockdown and miR-455-3p overexpression suppressed CD80 protein expression in SW1990 and Panc-1 cells. Knockdown of circ_102049 reduced (d) CD80; (e) TNF-α, IL-6, IL-1β, IL-8, and IL-17 expression; and (f) invasion ability in SW1990 and Panc-1 cells while inhibition of miR-455-3p in the meantime reversed it. The results were measured and expressed as . , vs. the si-NC+inhibitor-NC group. ^▲, ^▲▲ vs. the si-circ_102049_#1+inhibitor-NC group.

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