Involvement of Dendritic Cells and Th17 Cells in Induced Tertiary Lymphoid Structures in a Chronic Beryllium Disease Mouse Model

Figure 1

Characterization of the inflammation in BAL fluid of CBD mice. (a) Quantification of FACS analysis of the indicated cell populations in BAL at 8 weeks after initial exposure (granulocytes (GR1⁺ cells), macrophages (autofluorescence and macrophage gate), DCs (CD11c⁺MHCII⁺), and CD3⁺ T cells). (b–e) Intracellular flow cytometry analysis of cytokine production of BAL cells that were restimulated with PMA and ionomycin in the presence of Golgi-stop. CD3⁺ T cells were gated and analysed for their CD4/CD8 profile (b), which was quantified (c). CD3⁺CD4⁺ T cells were gated and analysed for intracellular cytokines (d), which were quantified (e). (f) Cytokine levels in BAL fluid, as determined by ELISA. Mice ( per group) were treated with PBS (controls, white bars) or Be/BeO (black bars). Mann-Whitney test: . Data are representative for 2 experiments performed.

(a)

(b)

(c)

(d)

(e)

(f)