Involvement of Dendritic Cells and Th17 Cells in Induced Tertiary Lymphoid Structures in a Chronic Beryllium Disease Mouse Model

Figure 2

Identification of nibbling cells around beryllium particles and formation of focal dens infiltrates. (a–c) Cytospin analysis of BAL cells from CBD mice, analysed by hematoxylin and eosin (H&E) staining 8 weeks after initial exposure (a); immunohistochemical staining for CD11c⁺ alveolar macrophages or DCs (red) (b); and for CD4⁺ T cells (red) (c). (d–i) Hematoxylin and eosin (H&E) analysis of lung sections. Staining of PBS-treated mice (d); Be-BeO-treated CBD mice following BAL collection (e–g) or without prior BAL collection, showing histiocytic and focal dense infiltrates. Black dots are Be particles, and brown gray dots are BeO particles. Arrows indicate the macrophage histiocytes, which were not present when BAL was performed. Data are representative for 3-5 mice analysed in each group. Data are expressed as the . Bars are 20 μm (a, b, c), 50 μm (f), 100 μm (h), and 500 μm (d, e).