Contribution of Thrombospondin-1 and -2 to Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome

Figure 5

The impact of TSP-2 on pulmonary vascular permeability in the ARDS mouse model. (a–l) Evans blue (EB) staining is shown as an index of pulmonary vascular permeability in lung sections collected from vehicle, TSP-1, and TSP-2-overexpressing mice. (m) Normal mice. (n) Percentage of EB leakage in pulmonary tissue compared to normal control. EB staining was visualized using red laser excitation at 405 nm OD. Blue color represents no EB leakage into the pulmonary tissue, red color depicts high EB leakage due to enhanced vascular permeability, and pink color shows medium vascular permeability. The arrows point to the site of EB leakage. and . (n) Evans blue leakage in pulmonary tissue (μg/g) is shown as . value of < 0.05 represents significant difference. ^a compared with normal control; ^b compared with the vehicle group; ^c compared with the TSP-1 transfected group ( per group).