Sphingosine Kinase 1 Plays an Important Role in Atorvastatin-Mediated Anti-Inflammatory Effect against Acute Lung Injury

Figure 3

The endothelial cell isolation from WT and SphK1^-/- mouse lung and identification. The 8-week WT and SphK1^-/- mice were sacrificed, and lungs were harvested as described in Materials and Methods. (a) The endothelial cells of WT mouse lung were purified by flow cytometry (CD31⁺/CD45^-). (b) The endothelial cells of SphK1^-/- mouse lung were isolated and purified (CD31⁺/CD45^-). (c) The purified WT and SphK1^-/- endothelial cells were cultured on glass slides, and morphological properties were compared, including size, lamellipodia, and junctional properties. (d) qPCR and (e) western blot were performed to determine the sphingosine kinase 1 mRNA and protein level in purified endothelial cells.

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