Sphingosine Kinase 1 Plays an Important Role in Atorvastatin-Mediated Anti-Inflammatory Effect against Acute Lung Injury

<div>JNK plays a critical role in sphingosine kinase 1-mediated inflammatory response to cyclic stretch. The isolated ECs were seeded on Flexcell plates overnight in the presence of the indicated inhibitors. After cyclic stretching (CS) (18% elongation, 0.5 Hz, 4 hours) as described in Figure <a href="../fig4/">4</a>, the medium was harvested for ELISA. (a) IL6 and KC assay showed that SphK1 knockout significantly enhanced CS-mediated IL6 and KC generation. The SphK1 knockout-mediated IL6 generation enhancement was not significantly abolished by PD98059 but partially by SB203580 and completely by JNK inhibitor II. (b) The SphK1 knockout-mediated KC generation enhancement was not significantly abolished by PD98059 and SB203580, but completely by JNK inhibitor II (<span class="nowrap"><svg height="10.7395pt" id="M122" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -10.1025 50.9184 10.7395" width="50.9184pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 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Mediators of Inflammation

fig6

Figure 6

Figure 6: Sphingosine Kinase 1 Plays an Important Role in Atorvastatin-Mediated Anti-Inflammatory Effect against Acute Lung Injury 