17β-Estradiol Attenuates LPS-Induced Macrophage Inflammation In Vitro and Sepsis-Induced Vascular Inflammation In Vivo by Upregulating miR-29a-5p Expression

Figure 1

E2 alleviates vascular inflammation in sepsis mice induced by CLP. (a) Immunofluorescence staining of SM22-α, MAC-2, and the nucleus on the vascular tissues of the control (NC), CLP, and CLP+E2 groups. Red and green stainings indicate SM22-α and MAC-2, respectively. Blue staining indicates the nucleus. . (b, c) IL-1β and NLRP3 expression in NC-, CLP-, and CLP+E2-treated vascular tissues was detected by qRT-PCR 24 h after CLP. Relative expression of IL-1β and NLRP3 mRNA was presented after normalizing to GAPDH. in each group. vs. the NC group. ^### vs. the CLP group. (d) IL-1β and NLRP3 expression in NC-, CLP-, and CLP+E2-treated vascular tissues was determined by Western blotting 24 h after CLP. Left panel: representative blot from five independent experiments. Right panel: quantitation of IL-1β and NLRP3 normalized to β-actin. vs. the NC group. ^### vs. the CLP group. (e) IL-1β in plasma was measured by ELISA. in each group. vs. the NC group. ^### vs. the CLP group.

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