17β-Estradiol Attenuates LPS-Induced Macrophage Inflammation In Vitro and Sepsis-Induced Vascular Inflammation In Vivo by Upregulating miR-29a-5p Expression

Figure 6

Depletion of miR-29a-5p promotes LPS-induced inflammation response. (a) qRT-PCR detected the mRNA expression of IL-1β and NLRP3 in RAW 264.7 cells transfected with the miR-29a-5p inhibitor or inhibitor-ctl and then treated with or without LPS (1 μg/ml) for 24 h. Relative expression of IL-1β and NLRP3 mRNA was presented after normalizing to GAPDH (; ). vs. the inhibitor-ctl group. ^### vs. the inhibitor-ctl+LPS group. (b) Western blotting detected IL-1β and NLRP3 expression in RAW 264.7 cells transfected with the miR-29a-5p inhibitor or inhibitor-ctl and then treated with or without LPS (1 μg/ml) for 24 h. Left panel: representative blot from three independent experiments. Right panel: quantitation of IL-1β and NLRP3 normalized to β-actin. vs. the inhibitor-ctl group. ^### vs. the inhibitor-ctl+LPS group.

(a)

(b)