Endangered Lymphocytes: The Effects of Alloxan and Streptozotocin on Immune Cells in Type 1 Induced Diabetes

<div>STZ has short-term diabetogenic impact on the population of B lymphocytes in the spleen. T1D was induced as described in Methods, and (a) spleens from the CT, ALX-, and STZ-treated groups were collected on the 15<sup>th</sup> day, and the total spleen cells were characterized according to the cell surface markers CD3, CD4, CD8, and CD19 by flow cytometry. (b) Spleen cells from each group were also stained with CFSE, incubated with medium, Con A (0.5 and 1 <i>μ</i>g/mL—final concentration), for 72 hours, and had T cell proliferation evaluated by flow cytometry. (c) Representative microphotographs of 3 <i>μ</i>m sections of spleen tissue stained with H&amp;E (scale 200 <i>μ</i>m). 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Mediators of Inflammation

fig5

Figure 5

Figure 5: Endangered Lymphocytes: The Effects of Alloxan and Streptozotocin on Immune Cells in Type 1 Induced Diabetes 