Hypoxia-Inducible Factor-1α in Macrophages, but Not in Neutrophils, Is Important for Host Defense during Klebsiella pneumoniae-Induced Pneumosepsis

Figure 1

HIF1α is important for glucose metabolism and TNF production of alveolar macrophages. HIF1α protein expression in AMs derived via BAL from naïve LysM-cre × Hif1α^fl/fl mice (cKO) and littermate controls (WT) treated with IOX2 for 24 hours (a). Lactate (b) and cytokine (TNF and IL-6) production (c) by AMs stimulated in vitro with LPS or left untreated for 24 hours. Data are shown as bar graphs showing mean with standard error of the mean from 6 technical replicates of pooled AMs from 7 mice per group. Dotted line indicates the reliable lower limit of detection of the cytokine assays. Lactate production by LysM-cre × Hif1α^fl/fl AMs was compared to that of control AMs (Hif1α^fl/fl) of mice using -tests. TNF and IL-6 production of LPS-stimulated cKO AMs and control AMs was compared using the Mann-Whitney test. ; .

(a)

(b)

(c)