Hypoxia-Inducible Factor-1α in Macrophages, but Not in Neutrophils, Is Important for Host Defense during Klebsiella pneumoniae-Induced Pneumosepsis

Figure 2

Macrophage HIF1α is important for host defense against K. pneumoniae in the lung. Bacterial loads (CFU/ml) in the lungs of LysM-cre × Hif1α^fl/fl mice and littermate controls 12 and 40 hours after intranasal inoculation with ~10⁴ CFU K. pneumoniae (a) or in distant organs 40 hours after inoculation (b). Data are shown as box-and-whisker diagrams of 13-16 mice per group from two independent experiments for each time point. Bacterial loads (CFU/ml) in the lungs of Mrp8-cre × Hif1α^fl/fl mice and littermate controls 12 and 40 hours after intranasal inoculation with ~10⁴ CFU K. pneumoniae (c) or in distant organs 40 hours after inoculation (d). Data are shown as box-and-whisker diagrams of 7-8 mice per group at each time point. Bacterial loads of the LysM-cre × Hif1α^fl/fl mice were compared to those of littermate control (Hif1α^fl/fl) mice using the Mann-Whitney test. ; ; .

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