Hypoxia-Inducible Factor-1<i>α</i> in Macrophages, but Not in Neutrophils, Is Important for Host Defense during <i>Klebsiella pneumoniae</i>-Induced Pneumosepsis

<div>HIF1<i>α</i> deficiency affects TNF production capacity of AMs and IMs. Percentage TNF-positive cells and the mean fluorescent intensity (MFI) of the TNF staining of AMs (a) and IMs (b) from LysM-cre × <i>Hif1α</i><sup>fl/fl</sup> (Cre+) and control mice (WT) after stimulation of lung suspensions for 2.5 hours with heat-killed <i>K. pneumoniae</i> (K. pneu) or medium control (MED). Data are shown as bar graphs showing mean with standard error of the mean from 10 mice per group. MFIs and percentage positive cells were compared using Student’s <span class="nowrap"><svg height="8.02022pt" id="M15" style="vertical-align:-0.2063999pt" version="1.1" viewbox="-0.0498162 -7.81382 4.54925 8.02022" width="4.54925pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M324 430H196L233 583L223 592L145 529L120 430H54L29 396L31 388H111L56 126C33 15 54 -12 77 -12C137 -12 214 57 250 95L233 119C208 92 155 59 138 59C126 59 120 70 131 125L186 390L298 394L324 430Z"></path></g></svg>-</span>tests followed by the Holm-Sidak multiple comparison test. <span class="nowrap"><svg height="10.7395pt" id="M16" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -10.1025 50.9184 10.7395" width="50.9184pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 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Mediators of Inflammation

fig4

Figure 4

Figure 4: Hypoxia-Inducible Factor-1<i>α</i> in Macrophages, but Not in Neutrophils, Is Important for Host Defense during <i>Klebsiella pneumoniae</i>-Induced Pneumosepsis 

Figure 4 | Hypoxia-Inducible Factor-1α in Macrophages, but Not in Neutrophils, Is Important for Host Defense during Klebsiella pneumoniae-Induced Pneumosepsis 