Hypoxia-Inducible Factor-1α in Macrophages, but Not in Neutrophils, Is Important for Host Defense during Klebsiella pneumoniae-Induced Pneumosepsis

Figure 5

HIF1α deficiency affects glucose metabolism but not mitochondrial mass of AMs and IMs. Glucose uptake after 2.5 hours of stimulation with heat-killed K. pneumoniae (K. pneu) or medium control (MED), as measured by the MFI of 2NBDG, of AMs and IMs within lung suspensions of LysM-cre × Hif1α^fl/fl mice and littermate controls (a). Mitochondrial mass as measured by the MFI of the MitoTracker Green probe in AMs and IMs of LysM-cre × Hif1α^fl/fl mice and littermate control after 3 hours of incubation in medium (b). As a negative control, lung suspensions were kept at 4°C. Data are shown as bar graphs showing mean with standard error of the mean of 10 mice per group for the glucose uptake assay and 6 mice per group for the mitochondrial mass. Groups were compared using Student’s -tests with the Holm-Sidak multiple comparison test where appropriate. ; ; .

(a)

(b)