BMSC-Derived Exosomes Ameliorate Osteoarthritis by Inhibiting Pyroptosis of Cartilage via Delivering miR-326 Targeting HDAC3 and STAT1//NF-κB p65 to Chondrocytes

Figure 2

BMSC-Exos inhibit the pyroptosis and maintain chondrocyte homeostasis of OA chondrocytes. (a) Incubation of BMSC-Exos reversed the slow proliferation of chondrocytes induced by OA; (b) incubation of BMSC-Exos reversed the reduction of chondrocyte migration induced by OA; (c) incubation of BMSC-Exos reversed the reduction of chondrogenic characteristic genes (COL2A1, SOX9, Agg, and Prg4) mRNA expression in chondrocytes induced by OA; (d) incubation of BMSC-Exos inhibited the increase of proinflammatory cytokines in chondrocyte culture medium induced by OA; (e) immunofluorescence results showed that BMSC-Exos incubation inhibited the OA-induced increased distribution of GSDMD and cl-Caspase-1 in chondrocytes; (f) BMSC-Exos incubation inhibited the OA-induced increased of Caspase-1 activity in chondrocytes; (g) results of Western blot showed that BMSC-Exos incubation inhibited the OA-induced increased expression of pyroptosis-related proteins (NLRP3, ASC, GSDMD, Caspase-1, IL-1β, and IL-18) in chondrocytes; .

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