BMSC-Derived Exosomes Ameliorate Osteoarthritis by Inhibiting Pyroptosis of Cartilage via Delivering miR-326 Targeting HDAC3 and STAT1//NF-κB p65 to Chondrocytes

Figure 6

Inhibition of HDAC3 or STAT1/NF-κB p65 signaling pathway counteracts pyroptosis induced by silence of miR-326 in chondrocytes. (a) Immunofluorescence results showed that inhibition of HDAC3 or STAT1/NF-κB p65 could counteract the increase of GSDMD and cleaved Caspase-1 induced by miR-326 inhibitor in chondrocytes; (b) inhibition of HDAC3 or STAT1/NF-κB p65 could counteract the increase of Caspase-1 activity induced by miR-326 inhibitor in chondrocytes; (c) inhibition of HDAC3 or STAT1/NF-κB p65 could counteract the increase of proinflammatory factors (IL-1β, IL-18, IL-6, and TNF-α) induced by miR-326 inhibitor in chondrocytes; (d) Western blot results showed that inhibition of HDAC3 or STAT1/NF-κB p65 could counteract the increase level of pyroptosis-related proteins (NLRP3, ASC, GSDMD, Caspase-1, IL-1β, and IL-18) induced by miR-326 inhibitor in chondrocytes; , .

(a)

(b)

(c)

(d)