Research Article
Long Noncoding RNA HCG9 Promotes Osteosarcoma Progression through RAD51 by Acting as a ceRNA of miR-34b-3p
Figure 4
miR-34b-3p overexpression attenuated HCG9-stimulated proliferation, migration, and invasion of osteosarcoma cells. (a) CCK-8 assay of cell proliferation in the control, HCG9, and HCG9+miR-34b-3p groups. (b) Colony formation and (c) cell counts in the control, HCG9, and HCG9+miR-34b-3p groups. (d) Representative images and (e) migrated and invaded cell numbers in the control, HCG9, and HCG9+miR-34b-3p groups. (f) Cell cycle analysis of the control, HCG9, and HCG9+miR-34b-3p groups. Scale bar: 100 μm. (g) Protein expression of RAD51 and H2A.X (h) Apoptosis-related protein expressions (Bax, Bcl-2, Caspase-3, and p53) in the control, HCG9, and HCG9+miR-34b-3p groups. in each group. vs. the control group. # vs. the HCG9 group.
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