The lncRNA H19/miR-766-3p/S1PR3 Axis Contributes to the Hyperproliferation of Keratinocytes and Skin Inflammation in Psoriasis via the AKT/mTOR Pathway

Figure 3

MiR-766-3p negatively regulates lncRNA H19. (a) Prediction of miR-766-3p by LncBase v.2 and microarray. (b) Expression level of lncRNA H19 in M5-induced HaCaT cell. (c) Fluorescence in situ hybridization showed the localization of lncRNA H19 in psoriasis tissue. Scale bar: 50 μm. (d) Expression level of lncRNA H19 in HaCaT cells by qRT-PCR. (e-f) HaCaT cells were transfected with PGMLV-H19/NC; lncRNA H19 expressions were detected with qRT-PCR (e); miR-766-3p expressions were detected with qRT-PCR (f). (g) The partial binding sites between miR-766-3p and lncRNA H19 are shown. (h) Luciferase activity was detected in HEK293 cells cotransfected with miR-766-3p mimics/NC and WT and MUT H19. Data were shown as the mean ± SD, P <0.05, P<0.001. All the experiments were repeated at least three times.

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