Extracellular HMGB1 Induced Glomerular Endothelial Cell Injury via TLR4/MyD88 Signaling Pathway in Lupus Nephritis

Figure 7

TAK242 alleviated the GEC injury in MRL/lpr mice. (a) IF staining of TLR4 (red) and CD31 (green) in the renal glomeruli cells of control and LN groups. (b) IF staining of MyD88 (red) and CD31 (green) in the renal glomeruli cells of control and LN groups. (c) IHC of NF-κB/p65 in the renal glomeruli cells of control and LN groups. (d) IF staining of MyD88 (red) and CD31 (green) in the renal glomeruli cells of mice. (e) IHC of NF-κB/p65 in the renal glomeruli of mice. (f) TEM showed the glomerular endothelial glycocalyx of mice. (g) The level of SDC-1 in mouse plasma detected by ELISA. (h) IF staining of SDC-1 (red) and CD31 (green) in the renal glomeruli cells of mice. (i) IHC of VCAM-1 in the mouse renal glomeruli cells of mice. (j) The level of VCAM-1 in the mouse plasma assessed by ELISA. (k) The level of NO in the renal cortex of mice. vs. control group. GEC: glomerular endothelial cell; TAK: TAK242.

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