Lysophosphatidic Acid Induced Apoptosis, DNA Damage, and Oxidative Stress in Spinal Cord Neurons by Upregulating LPA4/LPA6 Receptors

<div>LPA effects on cell viability and cytotoxicity in spinal cord neurons. (a) Observation of primary spinal cord neurons with microscope (100x). (b) The expression of <i>β</i>-tubulin in spinal cord neurons were measured by Immunofluorescence (200x). (c, e) spinal cord neurons were treated with different concentrations of LPA for 24 h, the cell viability was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, the LDH release was measured by LDH assay kit. (d) Cell viability was observed by microscope (100x). 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Mediators of Inflammation

fig2

Figure 2

Figure 2: Lysophosphatidic Acid Induced Apoptosis, DNA Damage, and Oxidative Stress in Spinal Cord Neurons by Upregulating LPA4/LPA6 Receptors 