Retinoic Acid Receptor Gamma (RARγ) Promotes Cartilage Destruction through Positive Feedback Activation of NF-κB Pathway in Human Osteoarthritis

Figure 3

RARγ induced the damage of cartilage matrix in cartilage cells. (a–d) RARγ overexpression (a,b) and knockdown (c,d) C28/I2 cell lines were constructed and verified by western blot and qRT-PCR. (e,f) CCK8 assay was used to detect the effects of RARγ overexpression (e) and knockdown (f) on proliferation of C28/I2 cells. (g–j) qRT-PCR was used to detect the changes of ECM degradation (g), inflammation (h), differentiation, and collagen (i) related genes after RARγ overexpression in C28/I2 cells. (j) The effects of knockdown RARγ on related gene levels in C28/I2 cell lines were detected by qRT-PCR. (k,l) RARγ overexpression and knockdown protein expression of MMP9, CCL4, and ADAMTS5 were analyzed by western blot. Data were of three independent assays (; ; and ).

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