Retinoic Acid Receptor Gamma (RARγ) Promotes Cartilage Destruction through Positive Feedback Activation of NF-κB Pathway in Human Osteoarthritis

Figure 4

RARγ induces chondrocytes degradation by activating NF-κB signaling pathway. (a–c) Effects of RARγ overexpression (a,b) and knockdown (c) on related signaling pathways in C28/I2 cells. (d,e) RARγ was overexpressed in C28/I2 and treated with BMS-345541 (10 μm) for 4 hours. The activation of NF-κB signaling pathway and the expression of related genes were detected by qRT-PCR and western blot. (f,g) RARγ was knockdown in C28/I2 and treated with TNF-α (30 ng/mL) for 4 hours. Activation of NF-κB signaling pathway and expression of related genes were detected by qRT-PCR and western blot. Data were of three independent assays (; ; and ).

(a)

(b)

(c)

(d)

(e)

(f)

(g)