Phosphorylation at Ser 727 Increases STAT3 Interaction with PKCε Regulating Neuron–Glia Crosstalk via IL-6-Mediated Hyperalgesia In Vivo and In Vitro

Figure 3

Western blot analysis of the proteins related to activated neurocytes during inflammatory pain. Examples (a) and mean values (b–g) of c-Fos, GFAP, Iba-1, PKCε, STAT3, and IL-6 proteins in the spinal cord. Intrathecally injected T-5224 (c-Fos/AP-1 inhibitor, 500 μg/50 μL), minocycline (Mino, 100 μg/50 μL), and L-2-aminoadipic acid (LAA, 1 mg/50 μL) reduced c-Fos, GFAP, Iba-1, PKCε, and IL-6 protein levels in FCA-treated rats, whereas STAT3 expression did not change between groups (). Data were normalized against GAPDH and are expressed as ratios (%) of control. Data are shown as (–5). , ; , one-way ANOVA with Bonferroni tests.

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