Homoharringtonine Attenuates Dextran Sulfate Sodium-Induced Colitis by Inhibiting NF-κB Signaling

Figure 5

HHT reduces the activation of NFκB signaling in vitro and in vivo. (a–c) Cultured RAW264.7 cells were stimulated with 1 μg/mL LPS along with vehicle or 50 nM HHT for 1 hour. The level of p-p65 (a, b), p65 (a), and p-IκBα (a, c) was analyzed by Western blot. (d–f) Mice were treated with 3% DSS along with vehicle or 2 mg/kg HHT for seven days, and the colon tissues were harvested to determine the protein level of p-p65 (d, e) and p-IκBα (d, f) by Western blot. Five mice per group. Error bar represents . .

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