Homoharringtonine Attenuates Dextran Sulfate Sodium-Induced Colitis by Inhibiting NF-κB Signaling

Figure 6

HHT inhibits the M1 polarization of macrophages. (a, b) Peritoneal macrophages were incubated with 50 ng/mL recombinant mouse IL4 along with vehicle or 50 nM HHT for 24 hours. The mRNA level of Arg1 (a) and Ym1 (b) was analyzed by RT-qPCR. (c, d) Peritoneal macrophages were treated with 100 ng/mL LPS and 20 ng/mL IFNγ along with vehicle or 50 nM HHT for 2 hours. The mRNA level of Nos2 (c) and TNF-α (d) were analyzed by RT-qPCR. (e, f) Peritoneal macrophages were stimulated with 100 ng/mL LPS and 20 ng/mL IFNγ along with vehicle or 50 nM HHT for 15 min. The protein level of p-IκBα (e, f), p-AKT, p-JNK, p-p38, and p-ERK (e) was analyzed by Western blot. Error bar represents . ; ns: not significant.

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