IL-21/IL-21R Regulates the Neutrophil-Mediated Pathologic Immune Response during Chlamydial Respiratory Infection

Figure 2

Pulmonary neutrophil activities of IL-21R^-/- mice in response to Chlamydia muridarum (C. muridarum) lung infection. (a, b) The CD64 expressions (shaded histogram) with fluorescence minus one (FMO) control (solid lines) on pulmonary neutrophils from wild-type (WT) and IL-21 receptor deficient (IL-21R^-/-) mice were analyzed by flow cytometry based on gated neutrophils as described in Figure 1(c). And the percentages of positive cells at indicated times after infection were indicated. (c) The myeloperoxidase (MPO) activity in the lung tissues at days 0, 3, 7, and 14 p.i. (d, e) The mRNA expression of matrix metalloproteinase 8 (MMP8) and S100A8 after C. muridarum infection was detected by quantitative real-time PCR (qPCR). Data are represented as from per group, representative one of three independent experiments. Statistical significances of differences are determined by two-way ANOVA followed by Bonferroni’s multiple comparisons test. , , and .

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